Watch the 5-4-1 Shuffle #3 online guitar lesson by Andrew Ford from Bass Groove Survival Guide
Again, really make those 16ths swing and pay special attention to the note length and accents that create the shuffle. Notice how note length affects the bounce factor in the shuffle(ex). The more legato version is actually the technically correct execution of what is written, but in my opinion does not create the best groove. Lets break this pattern down.